21名才赛车手中有9人会驾驶全新规则下的赛车。2012年世界冠军乔治-洛伦佐将在新赛季重新使用99号，而在去年拿到第二个世界冠军的凯西-斯通纳则拿到象征荣誉的1号。
具体车手名单：
Pos |
No |
Rider |
Team |
Bike |
| 1 | 1 |
Casey Stoner |
Honda Team |
Honda |
| 2 | 4 |
Andrea Dovizioso |
Yamaha Tech 3 |
Yamaha |
| 3 | 5 |
Colin Edwards |
Forward Racing |
Suter |
| 4 | 6 |
Stefan Bradl |
LCR Honda MotoGP |
Honda |
| 5 | 8 |
Hector Barbera |
Pramac Racing Team |
Ducati |
| 6 | 9 |
Danilo Petrucci |
Ioda Racing Project |
Ioda |
| 7 | 11 |
Ben Spies |
Yamaha Factory Racing |
Yamaha |
| 8 | 13 |
Anthony West |
Speed Master |
ART |
| 9 | 14 |
Randy de Puniet |
Aspar Team MotoGP |
ART |
| 10 | 17 |
Karel Abraham |
Cardion AB Motoracing |
Ducati |
| 11 | 19 |
Alvaro Bautista |
Honda Gresini |
Honda |
| 12 | 20 |
Aleix Espargaro |
Aspar Team MotoGP |
ART |
| 13 | 22 |
Ivan Silva |
BQR |
BQR-FTR |
| 14 | 26 |
Dani Pedrosa |
Honda Team |
Honda |
| 15 | 35 |
Cal Crutchlow |
Yamaha Tech 3 |
Yamaha |
| 16 | 46 |
Valentino Rossi |
Ducati Team |
Ducati |
| 17 | 51 |
Michele Pirro |
Honda Gresini |
Ftr |
| 18 | 68 |
Yonny Hernandez |
BQR |
BQR-FTR |
| 19 | 69 |
Nicky Hayden |
Ducati Team |
Ducati |
| 20 | 77 |
James Ellison |
Paul Bird Racing |
ART |
| 21 | 99 |
Jorge Lorenzo |
Yamaha Factory Racing |
Yamaha |